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Hygieia's Chalice is one of the World Items collected by Ainz Ooal Gown during its golden years. It was kept in the Treasury initially until Ainz learned of Shalltear's betrayal was the work of an unknown enemy's World Item brainwashing her. Because of that, it prompted Ainz to arm the Floor Guardians with World Items so as to protect themselves from the effects of hostile World Item users. This item was given to one of Demiurge or Cocytus.
The item is yet to be shown, making its appearance unknown.
Considering the fact that it is classified as a World Item, it can be said that it was a very rare and/or useful or strong item.
- This is given to either Demiurge or Cocytus from the Treasury.
- In Greek Mythology, it is recorded as a bowl/cup with a snake articulated around it and was in the possession of Hygieia, the daughter of Asklepios, the god of healing and medicine.